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phrl tk 5boxb sp36  (Addgene inc)


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  • 93

    Structured Review

    Addgene inc phrl tk 5boxb sp36
    Phrl Tk 5boxb Sp36, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phrl+tk+5boxb+sp36/phRL+TK+5BoxB+sp+36+(Plasmid+%23115365)/pm35654044-325-11-17
    Average 93 stars, based on 5 article reviews
    phrl tk 5boxb sp36 - by Bioz Stars, 2026-09
    93/100 stars

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    Construct:


    Article Title: A pro-metastatic tRNA fragment drives Nucleolin oligomerization and stabilization of bound metabolic mRNAs
    Article Snippet: Purified Nucleolin protein was incubated with 5’ radiolabeled 5’-tRF Cys and/or in-vitro transcribed 5’-tRF Cys targets in binding buffer (20 mM HEPES, pH 7, 50 mM KCl, 1 mM DTT, 1 mM EDTA) at 4 °C for 10 min before 3% of Ficoll-400 (Sigma) was added and assembled Nucleolin complexes were resolved on a 10% TBE PAGE gel at 4 °C. .. pcDNA3-FLUC containing zero, two or five BoxB sites were constructed from phRL-TK-5BoxB-sp36 (a gift from W. Filipowicz; Addgene #115365) ( ) and pcDNA3-RLUC-POLIRES-FLUC (a gift from N. Sonenberg; Addgene #45642) ( ) using Gibson assembly. .. HA-mNcl and λNHA-mNcl were constructed by subcloning mNcl from pcDNA3-mNcl-FLAG into pCIneo-deltaHA and pCIneo-deltaNHA (gifts from W. Filipowicz; Addgene #115360, #115359) ( ).



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    93
    Addgene inc phrl tk 5boxb sp36
    Phrl Tk 5boxb Sp36, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phrl+tk+5boxb+sp36/phRL+TK+5BoxB+sp+36+(Plasmid+%23115365)/pm35654044-325-11-17
    Average 93 stars, based on 1 article reviews
    phrl tk 5boxb sp36 - by Bioz Stars, 2026-09
    93/100 stars
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